Identification of adulterants in a Chinese herbal medicine by LC-HRMS and LC-MS-SPE/NMR and comparative in vivo study with standards in a hypertensive rat model

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Identification of adulterants in a Chinese herbal medicine by LC-HRMS and LC-MS-SPE/NMR and comparative in vivo study with standards in a hypertensive rat model. / Kesting, Julie Regitze; Huang, JingQi; Sørensen, Dan.

I: Journal of Pharmaceutical and Biomedical Analysis, Bind 51, Nr. 3, 2010, s. 705-711.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kesting, JR, Huang, J & Sørensen, D 2010, 'Identification of adulterants in a Chinese herbal medicine by LC-HRMS and LC-MS-SPE/NMR and comparative in vivo study with standards in a hypertensive rat model', Journal of Pharmaceutical and Biomedical Analysis, bind 51, nr. 3, s. 705-711. https://doi.org/10.1016/j.jpba.2009.09.043

APA

Kesting, J. R., Huang, J., & Sørensen, D. (2010). Identification of adulterants in a Chinese herbal medicine by LC-HRMS and LC-MS-SPE/NMR and comparative in vivo study with standards in a hypertensive rat model. Journal of Pharmaceutical and Biomedical Analysis, 51(3), 705-711. https://doi.org/10.1016/j.jpba.2009.09.043

Vancouver

Kesting JR, Huang J, Sørensen D. Identification of adulterants in a Chinese herbal medicine by LC-HRMS and LC-MS-SPE/NMR and comparative in vivo study with standards in a hypertensive rat model. Journal of Pharmaceutical and Biomedical Analysis. 2010;51(3):705-711. https://doi.org/10.1016/j.jpba.2009.09.043

Author

Kesting, Julie Regitze ; Huang, JingQi ; Sørensen, Dan. / Identification of adulterants in a Chinese herbal medicine by LC-HRMS and LC-MS-SPE/NMR and comparative in vivo study with standards in a hypertensive rat model. I: Journal of Pharmaceutical and Biomedical Analysis. 2010 ; Bind 51, Nr. 3. s. 705-711.

Bibtex

@article{59d89ae0c3b811debda0000ea68e967b,
title = "Identification of adulterants in a Chinese herbal medicine by LC-HRMS and LC-MS-SPE/NMR and comparative in vivo study with standards in a hypertensive rat model",
abstract = "Based on anecdotal evidence of anti-hypertensive effect of Gold Nine Soft Capsules, an in vivo study of this complex Chinese {"}herbal-based{"} medicine was initiated. Dosage of the content of Gold Nine capsules in spontaneous hypertensive rats showed a remarkably good effect. This led to further investigation of the components of the preparation and eventual identification of three known anti-hypertensive drugs; amlodipine, indapamide and valsartan, which were not declared on the label. Compounds were rapidly identified using LC-HRMS and LC-MS-SPE/NMR, quantified by HPLC, and the in vivo activity of a combination of commercially purchased standards was shown to be equivalent to that of the capsule content. Adulteration of herbal remedies and dietary supplements with synthetic drugs is an increasing problem that may lead to serious adverse effects. LC-MS-SPE/NMR as a method for the rapid identification of such adulterants is highlighted in this case study.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Kesting, {Julie Regitze} and JingQi Huang and Dan S{\o}rensen",
note = "Keywords: Chinese herbal medicine; Synthetic adulterants; Liquid chromatography-mass spectrometry-solid phase extraction/nuclear magnetic resonans (LC-MS-SPE/NMR); In vivo study; Hypertension",
year = "2010",
doi = "10.1016/j.jpba.2009.09.043",
language = "English",
volume = "51",
pages = "705--711",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Identification of adulterants in a Chinese herbal medicine by LC-HRMS and LC-MS-SPE/NMR and comparative in vivo study with standards in a hypertensive rat model

AU - Kesting, Julie Regitze

AU - Huang, JingQi

AU - Sørensen, Dan

N1 - Keywords: Chinese herbal medicine; Synthetic adulterants; Liquid chromatography-mass spectrometry-solid phase extraction/nuclear magnetic resonans (LC-MS-SPE/NMR); In vivo study; Hypertension

PY - 2010

Y1 - 2010

N2 - Based on anecdotal evidence of anti-hypertensive effect of Gold Nine Soft Capsules, an in vivo study of this complex Chinese "herbal-based" medicine was initiated. Dosage of the content of Gold Nine capsules in spontaneous hypertensive rats showed a remarkably good effect. This led to further investigation of the components of the preparation and eventual identification of three known anti-hypertensive drugs; amlodipine, indapamide and valsartan, which were not declared on the label. Compounds were rapidly identified using LC-HRMS and LC-MS-SPE/NMR, quantified by HPLC, and the in vivo activity of a combination of commercially purchased standards was shown to be equivalent to that of the capsule content. Adulteration of herbal remedies and dietary supplements with synthetic drugs is an increasing problem that may lead to serious adverse effects. LC-MS-SPE/NMR as a method for the rapid identification of such adulterants is highlighted in this case study.

AB - Based on anecdotal evidence of anti-hypertensive effect of Gold Nine Soft Capsules, an in vivo study of this complex Chinese "herbal-based" medicine was initiated. Dosage of the content of Gold Nine capsules in spontaneous hypertensive rats showed a remarkably good effect. This led to further investigation of the components of the preparation and eventual identification of three known anti-hypertensive drugs; amlodipine, indapamide and valsartan, which were not declared on the label. Compounds were rapidly identified using LC-HRMS and LC-MS-SPE/NMR, quantified by HPLC, and the in vivo activity of a combination of commercially purchased standards was shown to be equivalent to that of the capsule content. Adulteration of herbal remedies and dietary supplements with synthetic drugs is an increasing problem that may lead to serious adverse effects. LC-MS-SPE/NMR as a method for the rapid identification of such adulterants is highlighted in this case study.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1016/j.jpba.2009.09.043

DO - 10.1016/j.jpba.2009.09.043

M3 - Journal article

C2 - 19850434

VL - 51

SP - 705

EP - 711

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 3

ER -

ID: 15405923